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Blood Group O→A Transformation by Chemical Ligation of Erythrocytes

Ryzhov, IM; Tuzikov, AB; Perry, H; Korchagina, EY; Bovin, NV
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http://hdl.handle.net/10292/13203
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Abstract
Agglutination of red blood cells (RBCs) remains the only practical method for routine use for ABH typing in clinical practice. However, exact mechanistic details of agglutination are not yet thoroughly studied. In this research, RBCs of blood group O were converted to blood group A through two approaches: by chemical ligation of the cells’ glycocalyx with synthetic blood group A tetrasaccharide, and by insertion of synthetic glycolipid carrying the same A antigen into the cells’ membranes. The O→A ligated RBCs and natural A RBCs showed comparable agglutination characteristics with antibodies. As expected, RBCs with inserted glycolipid showed lower agglutination scores. This approach could help cell biologists in site-specific and cell-friendly modification of glycocalyx by other ligands.
Keywords
ABH antigens; Carbohydrates; Erythrocytes; Glycocalyx; Glycolipids
Date
2018
Source
ChemBioChem, 20(2), 131-133.
Item Type
Journal Article
Publisher
John Wiley & Sons, Inc.
DOI
10.1002/cbic.201800289
Publisher's Version
https://onlinelibrary.wiley.com/doi/full/10.1002/cbic.201800289
Rights Statement
Free Access © 2019 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim

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