Blood Group O→A Transformation by Chemical Ligation of Erythrocytes

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aut.relation.endpage133
aut.relation.journalChemBioChemen_NZ
aut.relation.startpage131
aut.relation.volume20en_NZ
dark.contributor.authorRyzhov, IMen_NZ
dark.contributor.authorTuzikov, ABen_NZ
dark.contributor.authorPerry, Hen_NZ
dark.contributor.authorKorchagina, EYen_NZ
dark.contributor.authorBovin, NVen_NZ
dc.date.accessioned2020-03-13T02:26:55Z
dc.date.available2020-03-13T02:26:55Z
dc.date.copyright2018en_NZ
dc.date.issued2018en_NZ
dc.description.abstractAgglutination of red blood cells (RBCs) remains the only practical method for routine use for ABH typing in clinical practice. However, exact mechanistic details of agglutination are not yet thoroughly studied. In this research, RBCs of blood group O were converted to blood group A through two approaches: by chemical ligation of the cells’ glycocalyx with synthetic blood group A tetrasaccharide, and by insertion of synthetic glycolipid carrying the same A antigen into the cells’ membranes. The O→A ligated RBCs and natural A RBCs showed comparable agglutination characteristics with antibodies. As expected, RBCs with inserted glycolipid showed lower agglutination scores. This approach could help cell biologists in site-specific and cell-friendly modification of glycocalyx by other ligands.en_NZ
dc.identifier.citationChemBioChem, 20(2), 131-133.
dc.identifier.doi10.1002/cbic.201800289en_NZ
dc.identifier.issn1439-4227en_NZ
dc.identifier.issn1439-7633en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/13203
dc.publisherJohn Wiley & Sons, Inc.
dc.relation.urihttps://onlinelibrary.wiley.com/doi/full/10.1002/cbic.201800289en_NZ
dc.rightsFree Access © 2019 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectABH antigens; Carbohydrates; Erythrocytes; Glycocalyx; Glycolipids
dc.titleBlood Group O→A Transformation by Chemical Ligation of Erythrocytesen_NZ
dc.typeJournal Article
pubs.elements-id341982
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Design & Creative Technologies
pubs.organisational-data/AUT/Design & Creative Technologies/Engineering, Computer & Mathematical Sciences
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Applied Science
pubs.organisational-data/AUT/Health & Environmental Science/School of Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Design and Creative Technologies
pubs.organisational-data/AUT/PBRF/PBRF Design and Creative Technologies/PBRF ECMS
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HA Science 2018 PBRF
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