Distinct Microbiota Composition and Fermentation Products Indicate Functional Compartmentalization in the Hindgut of a Marine Herbivorous Fish

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aut.relation.endpage2509
aut.relation.issue8en_NZ
aut.relation.journalMolecular Ecologyen_NZ
aut.relation.startpage2494
aut.relation.volume31en_NZ
aut.researcherDrabsch, Julie
dc.contributor.authorPardesi, Ben_NZ
dc.contributor.authorRoberton, AMen_NZ
dc.contributor.authorLee, KCen_NZ
dc.contributor.authorAngert, ERen_NZ
dc.contributor.authorRosendale, DIen_NZ
dc.contributor.authorBoycheva, Sen_NZ
dc.contributor.authorWhite, WLen_NZ
dc.contributor.authorClements, KDen_NZ
dc.date.accessioned2022-06-08T02:21:53Z
dc.date.available2022-06-08T02:21:53Z
dc.date.copyright2022en_NZ
dc.date.issued2022en_NZ
dc.description.abstractMany marine herbivorous fishes harbour diverse microbial communities in the hindgut that can play important roles in host health and nutrition. Kyphosus sydneyanus is a temperate marine herbivorous fish that feeds predominantly on brown seaweeds. We employed 16S rRNA gene amplicon sequencing and gas chromatography to characterize microbial communities and their metabolites in different hindgut regions of six K. sydneyanus. Measurements were confined to three distal sections of the intestine, labelled III, IV and V from anterior to posterior. A total of 625 operational taxonomic units from 20 phyla and 123 genera were obtained. Bacteroidota, Firmicutes and Proteobacteria were the major phyla in mean relative abundance, which varied along the gut. Firmicutes (76%) was the most dominant group in section III, whereas Bacteroidota (69.3%) dominated section V. Total short-chain fatty acid (SCFA) concentration was highest in sections IV and V, confirming active fermentation in these two most distal sections. The abundance of Bacteroidota correlated with propionate concentration in section V, while Firmicutes positively correlated with formate in sections III and IV. Acetate levels were highest in sections IV and V, which correlated with abundance of Bacteroidota. Despite differences in gut microbial community composition, SCFA profiles were consistent between individual fish in the different hindgut regions of K. sydneyanus, although proportions of SCFAs differed among gut sections. These findings demonstrate functional compartmentalization of the hindgut microbial community, highlighting the need for regional sampling when interpreting overall microbiome function. These results support previous work suggesting that hindgut microbiota in marine herbivorous fish are important to nutrition in some host species by converting dietary carbohydrates into metabolically useful SCFAs.en_NZ
dc.identifier.citationMolecular Ecology, 31(8):2494-2509 2022
dc.identifier.doi10.1111/mec.16394en_NZ
dc.identifier.issn0962-1083en_NZ
dc.identifier.issn1365-294Xen_NZ
dc.identifier.urihttps://hdl.handle.net/10292/15204
dc.publisherJohn Wiley & Sons
dc.relation.urihttps://onlinelibrary.wiley.com/doi/10.1111/mec.16394
dc.rights© 2022 The Authors. Molecular Ecology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
dc.rights.accessrightsOpenAccessen_NZ
dc.subject16S rRNA; Algae; Digestion; Microbiota; Short-chain fatty acids
dc.titleDistinct Microbiota Composition and Fermentation Products Indicate Functional Compartmentalization in the Hindgut of a Marine Herbivorous Fishen_NZ
dc.typeJournal Article
pubs.elements-id450176
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Faculty of Health & Environmental Science
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Science
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Science/Environmental Science Department
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Science/Food Science & Microbiology Department
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HA Science 2018 PBRF
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