Mapping the Green-Lipped Mussel (Perna canaliculus) Microbiome: A Multi-Tissue Analysis of Bacterial and Fungal Diversity

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aut.relation.articlenumber76en_NZ
aut.relation.issue3en_NZ
aut.relation.journalCurrent Microbiologyen_NZ
aut.relation.volume79en_NZ
aut.researcherDrabsch, Julie
dc.contributor.authorLi, Sen_NZ
dc.contributor.authorYoung, Ten_NZ
dc.contributor.authorArcher, Sen_NZ
dc.contributor.authorLee, Ken_NZ
dc.contributor.authorSharma, Sen_NZ
dc.contributor.authorAlfaro, ACen_NZ
dc.date.accessioned2022-06-08T02:35:23Z
dc.date.available2022-06-08T02:35:23Z
dc.date.copyright2022en_NZ
dc.date.issued2022en_NZ
dc.description.abstractPoor health and mortality events of the commercially important and endemic New Zealand green-lipped mussel (Perna canaliculus) pose a threat to its industry. Despite the known importance of microbiomes to animal health and environmental resilience, the host-associated microbiome is unexplored in this species. We conducted the first baseline characterization of bacteria and fungi within key host tissues (gills, haemolymph, digestive gland, and stomach) using high-throughput amplicon sequencing of 16S rRNA gene and ITS1 region for bacteria and fungi, respectively. Tissue types displayed distinctive bacterial profiles, consistent among individuals, that were dominated by phyla which reflect (1) a fluid exchange between the circulatory system (gills and haemolymph) and surrounding aqueous environment and (2) a highly diverse digestive system (digestive gland and stomach) microbiota. Gammaproteobacteria and Campylobacterota were mostly identified in the gill tissue and haemolymph, and were also found in high abundance in seawater. Digestive gland and stomach tissues were dominated by common gut bacterial phyla, such as Firmicutes, Cyanobacteria, Proteobacteria, and Bacteroidota, which reflects the selectivity of the digestive system and food-based influences. Other major notable taxa included the family Spirochaetaceae, and genera Endozoicomonas, Psychrilyobacter, Moritella and Poseidonibacter, which were highly variable among tissue types and samples. More than 50% of fungal amplicon sequence variants (ASVs) were unclassified beyond the phylum level, which reflects the lack of studies with marine fungi. However, the majority of those identified were assigned to the phylum Ascomycota. The findings from this work provide the first insight into healthy tissue microbiomes of P. canaliculus and is of central importance to understanding the effect of environmental changes on farmed mussels at the microbial level.en_NZ
dc.identifier.citationCurrent Microbiology, 79, 76 (2022). https://doi.org/10.1007/s00284-021-02758-5
dc.identifier.doi10.1007/s00284-021-02758-5en_NZ
dc.identifier.issn0343-8651en_NZ
dc.identifier.issn1432-0991en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/15205
dc.languageengen_NZ
dc.publisherSpringeren_NZ
dc.relation.urihttps://link.springer.com/article/10.1007/s00284-021-02758-5
dc.rights© The Author(s) 2022. This article is licensed under a Creative Commons Attri bution 4.0 International License, which permits use, sharing, adapta tion, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.accessrightsOpenAccessen_NZ
dc.titleMapping the Green-Lipped Mussel (Perna canaliculus) Microbiome: A Multi-Tissue Analysis of Bacterial and Fungal Diversityen_NZ
dc.typeJournal Article
pubs.elements-id448165
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Faculty of Health & Environmental Science
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Science
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Science/Environmental Science Department
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Science/Food Science & Microbiology Department
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HA Science 2018 PBRF
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