Isolation of Polymorphic Microsatellite Loci in the New Zealand Endemic Sand-Binder, Ficinia spiralis (Cyperaceae)

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aut.relation.issue9en_NZ
aut.relation.journalApplications in Plant Sciencesen_NZ
aut.relation.volume5en_NZ
aut.researcherBuckley, Hannah
dc.contributor.authorHeugten, RAVen_NZ
dc.contributor.authorHale, MLen_NZ
dc.contributor.authorBryan, Sen_NZ
dc.contributor.authorGriensven, BVen_NZ
dc.contributor.authorSatter, Sen_NZ
dc.contributor.authorBrailsford, Len_NZ
dc.contributor.authorBuckley, HLen_NZ
dc.date.accessioned2019-10-03T21:36:21Z
dc.date.available2019-10-03T21:36:21Z
dc.date.copyright2017-09-01en_NZ
dc.date.issued2017-09-01en_NZ
dc.description.abstractPremise of the study: Ficinia spiralis (Cyperaceae) is a declining sand-binding sedge of ecological and cultural importance. Microsatellite primers were developed in F. spiralis to investigate how population genetic structure is related to the pronounced morphological, physiological, and ecological variation observed in this species. Methods and Results: A 454 shotgun-sequencing approach was used to generate 157,274 raw sequence reads, 536 of which contained microsatellites. After initial primer testing for 40 loci, 14 polymorphic loci were isolated, containing five to 27 alleles per locus. Ten of these loci also amplified in a congener, F. nodosa. Conclusions: These loci will enable the assessment of the population genetic structure of F. spiralis, improving our understanding of the population processes underlying the observed morphological, physiological, and ecological variation in this endemic species. As the first microsatellites developed in Ficinia, these loci are a valuable resource for population genetic studies within this genus.en_NZ
dc.identifier.citationApplications in Plant Sciences 2017 5(9): 1700039
dc.identifier.doi10.3732/apps.1700039en_NZ
dc.identifier.issn2168-0450en_NZ
dc.identifier.issn1537-2197en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/12878
dc.publisherBotanical Society of America
dc.relation.urihttps://bsapubs.onlinelibrary.wiley.com/doi/full/10.3732/apps.1700039
dc.rights© 2017 The Author(s). This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐ShareAlike License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and the content is offered under identical terms.
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectCyperaceae; Ficinia nodosa; Ficinia spiralis; pīngao; polyploidy; microsatellites
dc.titleIsolation of Polymorphic Microsatellite Loci in the New Zealand Endemic Sand-Binder, Ficinia spiralis (Cyperaceae)en_NZ
dc.typeJournal Article
pubs.elements-id315765
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Applied Science
pubs.organisational-data/AUT/Health & Environmental Science/School of Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HA Science 2018 PBRF
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