Videos relating to Fluorescent Function-Spacer-Lipid construct labeling allows for real-time in vivo imaging of cell migration and behaviour in zebrafish (Danio rerio)
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Video 1: 2 hours post injection imaging of the caudal vein plexus area of 52 hpf recipient zebrafish receiving 0.2 mg/ml FSL-FLRO4-I transformed WKM cells. In the video, a large slow-moving cell tumbles along the endothelial surface. Elongated oval shaped erythrocytes move at a fast speed.
Video 2: Embryos (50-52hpf) were injected with 0.125 mg/ml FSL-FLRO4-II-treated cells. Window one focused on the eye region.
Video 3: Embryos (50-52hpf) were injected with 0.125 mg/ml FSL-FLRO4-II-treated cells. Window two focused on the heart region.
Video 4: Embryos (50-52hpf) were injected with 0.125 mg/ml FSL-FLRO4-II-treated cells. Window three focused on the first half the yolk extension region.
Video 5: Embryos (50-52hpf) were injected with 0.125 mg/ml FSL-FLRO4-II-treated cells. Window four focused on the caudal half of the yolk extension and the anal regions.
Video 6: Embryos (50-52hpf) were injected with 0.125 mg/ml FSL-FLRO4-II-treated cells. Window five focused on the caudal haematopoietic tissue region.
Video 7: Embryos (50-52hpf) were injected with 0.125 mg/ml FSL-FLRO4-II-treated cells. Window six focused on the tail region.
Video 8: Temporal assessment of FSL-FLRO4-II labeled cells in the lower trunk area. The image was taken 2 hours post injection for the sham-injected fish.
Video 9: Temporal assessment of FSL-FLRO4-II labeled cells in the lower trunk area. The image was taken 2 hours post injection for the fish transplanted with FSL-FLRO4-II treated cells.
Video 10: Temporal assessment of FSL-FLRO4-II labeled cells in the lower trunk area. The image was taken 19 hours post injection for the sham-injected fish.
Video 11: Temporal assessment of FSL-FLRO4-II labeled cells in the lower trunk area. The image was taken 19 hours post injection for the fish transplanted with FSL-FLRO4-II treated cells.
Video 12: Temporal assessment of FSL-FLRO4-II labeled cells in the lower trunk area. The image was taken 43 hours post injection for the sham-injected fish.
Video 13: Temporal assessment of FSL-FLRO4-II labeled cells in the lower trunk area. The image was taken 43 hours post injection for the fish transplanted with FSL-FLRO4-II treated cells.