Fucoidan Extracted From New Zealand Undaria Pinnatifida – Physicochemical Comparison Against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Lines

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aut.relation.endpage25
aut.relation.issue12en_NZ
aut.relation.journalMarine Drugsen_NZ
aut.relation.startpage1
aut.relation.volume16en_NZ
aut.researcherLu, Jun
dc.contributor.authorHassouna, Aen_NZ
dc.contributor.authorLu, Jen_NZ
dc.contributor.authorMerien, Fen_NZ
dc.contributor.authorWhite, Wen_NZ
dc.contributor.authorWhite, Len_NZ
dc.date.accessioned2019-04-08T23:42:19Z
dc.date.available2019-04-08T23:42:19Z
dc.date.copyright2018en_NZ
dc.date.issued2018en_NZ
dc.description.abstractFucoidan, the complex fucose-containing sulphated polysaccharide varies considerably in structure, composition, and bioactivity, depending on the source, species, seasonality, and extraction method. In this study, we examined five fucoidans extracted from the same seaweed species Undaria pinnatifida but from different geological locations, and compared them to the laboratory-grade fucoidan from Sigma (S). The five products differed in molecular composition. The amount of over 2 kDa low molecular weight fraction (LMWF) of the New Zealand crude fucoidan (S1) was larger than that of S, and this fraction was unique, compared to the other four fucoidans. The difference of molecular compositions between S and S1 explained our previous observation that S1 exhibited different anticancer profile in some cancer cell lines, compared with S. Since we observed this unique LMWF, we compared the cytotoxic effects of a LMWF and a high molecular weight fucoidan (HMWF) in two breast cancer cell lines—MCF-7 and MDA-MB-231. Results indicated that the molecular weight is a critical factor in determining the anti-cancer potential of fucoidan, from the New Zealand U. pinnatifida, as the LMWF exhibited a dose-dependent inhibition on the proliferation of breast cancer cells, significantly better than the HMWF, in both cell lines. A time-dependent inhibition was only observed in the MCF-7. Induction of caspase-dependent apoptosis was observed in the MDA-MB-231 cells, through the intrinsic apoptosis pathway alone, or with the extrinsic pathway. LMWF stimulated a dose-dependent NOS activation in the MDA-MB-231 cells. In conclusion, the fucoidan extracted from the New Zealand U. pinnatifida contains a unique LMWF, which could effectively inhibit the growth of breast cancer cell lines. Therefore, the LMWF from New Zealand U. pinnatifida could be used as a supplement cancer treatment.
dc.identifier.citationMarine Drugs, 16(12), 461. doi:10.3390/md16120461
dc.identifier.doi10.3390/md16120461en_NZ
dc.identifier.issn1660-3397en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/12427
dc.publisherMDPI AGen_NZ
dc.relation.urihttps://www.mdpi.com/1660-3397/16/12/461
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectFucoidan; Chemical composition; Molecular weight; Breast cancer cell lines; Nutraceutical
dc.titleFucoidan Extracted From New Zealand Undaria Pinnatifida – Physicochemical Comparison Against Five Other Fucoidans: Unique Low Molecular Weight Fraction Bioactivity in Breast Cancer Cell Linesen_NZ
dc.typeJournal Article
pubs.elements-id349656
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Applied Science
pubs.organisational-data/AUT/Health & Environmental Science/Interprofessional Health
pubs.organisational-data/AUT/Health & Environmental Science/School of Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HA Science 2018 PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
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