Modular Assembly and Optimization of an Artificial Esterase from Functionalised Surfactants

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aut.relation.issue20
aut.relation.journalChemCatChem
aut.relation.volume16
dc.contributor.authorMatich, Olivia
dc.contributor.authorNaiya, Mohinder Maheshbhai
dc.contributor.authorSalam, Joanne
dc.contributor.authorTiban Anrango, Bryan Andres
dc.contributor.authorChen, Jack L‐Y
dc.date.accessioned2024-11-11T23:53:15Z
dc.date.available2024-11-11T23:53:15Z
dc.date.issued2024-06-21
dc.description.abstractA strategy for the screening and optimization of an artificial esterase is presented that utilizes the self‐assembly of amphiphilic molecules. Unlike conventional approaches that rely on the attachment of key functional groups onto molecular scaffolds or surfaces, the modular assembly of amphiphiles allows a large number of catalytic combinations to be investigated with minimal synthetic effort. In this study, iterative combinatorial screens led to an optimized esterase comprising amphiphiles that act as a nucleophilic catalyst, an oxyanion hole and a metal ion chelator. Cooperativity is observed between the functional headgroups of the amphiphiles, an effect that is diminished when co‐assembled with non‐functionalized surfactants. Assessment of the catalytic efficiency (kcat/KM) of our optimized catalysts against recently reported artificial esterases shows comparable efficiency, indicating that efficient catalysis is possible with dynamic self‐assembled systems despite the absence of pre‐defined rigid binding pockets.
dc.identifier.citationChemCatChem, ISSN: 1867-3880 (Print); 1867-3899 (Online), Wiley, 16(20). doi: 10.1002/cctc.202400945
dc.identifier.doi10.1002/cctc.202400945
dc.identifier.issn1867-3880
dc.identifier.issn1867-3899
dc.identifier.urihttp://hdl.handle.net/10292/18277
dc.languageen
dc.publisherWiley
dc.relation.urihttps://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cctc.202400945
dc.rights© 2024 The Author(s). ChemCatChem published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
dc.rights.accessrightsOpenAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subject4004 Chemical Engineering
dc.subject40 Engineering
dc.subject0302 Inorganic Chemistry
dc.subject0306 Physical Chemistry (incl. Structural)
dc.subject0904 Chemical Engineering
dc.subjectOrganic Chemistry
dc.subject4004 Chemical engineering
dc.titleModular Assembly and Optimization of an Artificial Esterase from Functionalised Surfactants
dc.typeJournal Article
pubs.elements-id566407
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