Fluorophore-kodecytes - fluorescent function-spacer-lipid (FSL) modified cells for in vitro and in vivo analyses

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aut.researcherHenry, Stephen Micheal
dc.contributor.authorBlake, D
dc.contributor.authorLan, A
dc.contributor.authorLove, D
dc.contributor.authorBovin, N
dc.contributor.authorHenry, S
dc.date.accessioned2011-09-28T01:45:16Z
dc.date.available2011-09-28T01:45:16Z
dc.date.copyright2010-06-01
dc.date.issued2010-06-01
dc.description.abstractMost live cells are naturally poorly visible and so various secondary techniques, such as staining (including fluorescent tagging), are used to visualize them. However, a major limitation of most staining techniques is that they commonly compromise cell vitality and/or functionality because the stain either covalently attaches to functional molecules or has toxic interactions. In contrast, FSL (function-spacer-lipid) constructs are designed to be dispersible in biological media, they can insert into cell membranes, and a range of synthetic molecules can be attached, such as fluorophores, without affecting the cells functionality or vitality (1–3). Two constructs for fluorescent tagging of live cells were investigated. In the first, FSL incorporated FITC as its functional moiety (FSLFITC), and the second approach used biotin (FSL-biotin), which was then secondarily reacted with fluorophore labeled avidin. Both approaches were used to successfully label a variety of living cells including murine embryos, spermatozoa, epithelial and endometrial cells. FSL-FITC was additionally used to stain the digestive tract and circulation of zebra fish embryos. Acknowledgement: Supported by KODE Biotech Ltd (kodebiotech.com).
dc.identifier.citationFederation of European Biochemical Societies(FEBS) Journal, Special Issue: Abstracts of the 35th FEBS Congress, Gothenburg, Sweden, vol.277(Suppl. 1), pp.199
dc.identifier.doi10.1111/j.1742-4658.2010.07680.x
dc.identifier.issn1742-464X (print) 1742-4658 (online)
dc.identifier.urihttps://hdl.handle.net/10292/2144
dc.publisherJohn Wiley & Sons Ltd
dc.relation.urihttp://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2010.07680.x/pdf
dc.rightsCopyright © 2010 John Wiley & Sons. All rights reserved. Authors retain the right to place his/her pre-publication version of the work on a personal website or institutional repository. This article may not exactly replicate the final version published in (please see citation) as it is not a copy of this record. An electronic version of this article can be found online at: (Please see Publisher’s Version)
dc.rights.accessrightsOpenAccess
dc.subjectRed
dc.subjectBlood
dc.subjectCells
dc.titleFluorophore-kodecytes - fluorescent function-spacer-lipid (FSL) modified cells for in vitro and in vivo analyses
dc.typeConference Contribution
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/PBRF Researchers
pubs.organisational-data/AUT/PBRF Researchers/Health & Environmental Sciences PBRF Researchers
pubs.organisational-data/AUT/PBRF Researchers/Health & Environmental Sciences PBRF Researchers/HES Applied Science
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