Bioremediation of Oily Hypersaline Soil via Autochthonous Bioaugmentation with Halophilic Bacteria and Archaea

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aut.relation.articlenumber171279
aut.relation.journalScience of the Total Environment
aut.relation.startpage171279
aut.relation.volume922
dc.contributor.authorLee, KC
dc.contributor.authorArcher, SDJ
dc.contributor.authorKansour, MK
dc.contributor.authorAl-Mailem, DM
dc.date.accessioned2024-07-08T04:24:51Z
dc.date.available2024-07-08T04:24:51Z
dc.date.issued2024-02-28
dc.description.abstractKuwaiti hypersaline soil samples were contaminated with 5 % (w/w) weathered Kuwaiti light crude oil and bioaugmented with autochthonous halophilic hydrocarbonoclastic archaeal and bacterial strains, two each, individually and as consortia. Residual oil contents were determined, and microbial communities were analyzed by culture-dependent and culture-independent approaches initially and seasonally for one year. After one year of the bioremediation process, the mean oil degradation rate was similar across all treated soils including the controlled unbioaugmented one. Oil hydrocarbons were drastically reduced in all soil samples with values ranging from 82.7 % to 93 %. During the bioremediation process, the number of culturable oil-degrading bacteria increased to a range of 142 to 344 CFUx104 g−1 after 12 months of bioaugmentation. Although culture-independent analysis showed a high proportion of inoculants initially, none could be cultured throughout the bioremediation procedure. Within a year, microbial communities changed continually, and 33 species of halotolerant/halophilic hydrocarbonoclastic bacteria were isolated and identified belonged mainly to the three major bacterial phyla Actinobacteria, Proteobacteria, and Firmicutes. The archaeal phylum Halobacterota represented <1 % of the microbial community's relative abundance, which explains why none of its members were cultured. Improving the biodegradability of an already balanced environment by autochthonous bioaugmentation is more involved than just adding the proper oil degraders. This study emphasizes the possibility of a relatively large resistant population, a greater diversity of oil-degrading microorganisms, and the highly selective impacts of oil contamination on hypersaline soil bacterial communities.
dc.identifier.citationScience of the Total Environment, ISSN: 0048-9697 (Print); 1879-1026 (Online), Elsevier BV, 922, 171279-. doi: 10.1016/j.scitotenv.2024.171279
dc.identifier.doi10.1016/j.scitotenv.2024.171279
dc.identifier.issn0048-9697
dc.identifier.issn1879-1026
dc.identifier.urihttp://hdl.handle.net/10292/17760
dc.languageeng
dc.publisherElsevier BV
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0048969724014189
dc.rights.accessrightsOpenAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.uri© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
dc.subjectAutochthonous bioaugmentation
dc.subjectBioremediation
dc.subjectHalophiles
dc.subjectHydrocarbonoclastic
dc.subjectHypersaline soil
dc.subject3107 Microbiology
dc.subject31 Biological Sciences
dc.subject4103 Environmental Biotechnology
dc.subject41 Environmental Sciences
dc.subjectEnvironmental Sciences
dc.subject.meshArchaea
dc.subject.meshBiodegradation, Environmental
dc.subject.meshSoil
dc.subject.meshSoil Microbiology
dc.subject.meshOils
dc.subject.meshBacteria
dc.subject.meshPetroleum
dc.subject.meshHydrocarbons
dc.subject.meshSoil Pollutants
dc.subject.meshBacteria
dc.subject.meshArchaea
dc.subject.meshHydrocarbons
dc.subject.meshOils
dc.subject.meshSoil
dc.subject.meshSoil Pollutants
dc.subject.meshSoil Microbiology
dc.subject.meshPetroleum
dc.subject.meshBiodegradation, Environmental
dc.subject.meshArchaea
dc.subject.meshBiodegradation, Environmental
dc.subject.meshSoil
dc.subject.meshSoil Microbiology
dc.subject.meshOils
dc.subject.meshBacteria
dc.subject.meshPetroleum
dc.subject.meshHydrocarbons
dc.subject.meshSoil Pollutants
dc.titleBioremediation of Oily Hypersaline Soil via Autochthonous Bioaugmentation with Halophilic Bacteria and Archaea
dc.typeJournal Article
pubs.elements-id541962
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