Putative Involvement of Adrenergic Receptors in Regulation of Mussel (Perna Canaliculus) Larval Settlement

Date
2015
Authors
Young, T
Alfaro, AC
Sánchez-Lazo, C
Robertson, J
Supervisor
Item type
Journal Article
Degree name
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor and Francis Ltd.
Abstract

Abstract: Settlement responses were investigated for mussel (Perna canaliculus) larvae after exposure to catecholamines and their precursor metabolites. Settlement and mortality assays were conducted in Petri plates with chemical treatments (L-phenylalanine, L-tyrosine, L-DOPA, dopamine hydrochloride and epinephrine at various concentrations) and controls. The proteinogenic amino acids L-phenylalanine and L-tyrosine were both effective inducers (~65%) of larval settlement at 10−5 mol l−1 compared with controls (4%). Exposure of larvae to L-DOPA, dopamine and epinephrine resulted in maximum settlement induction (50, 60 and 51%, respectively) at 10−5 mol l−1. Larval mortalities were low (comparable to controls) across all concentrations of L-phenylalanine and L-tyrosine treatments, whereas high mortalities (>60%) were observed for L-DOPA, dopamine and epinephrine at concentrations ≥ 10−4 mol l−1. Our results indicate that P. canaliculus larval settlement is under endogenous regulation by a catecholaminergic mechanism. Furthermore, the inductive effects of all tested metabolites in the epinephrine biosynthesis pathway point to a putative involvement of adrenergic-type receptors in the regulation of larval settlement in this mussel species.

Description
Keywords
Catecholamines , Dopamine , Epinephrine , Green-lipped mussels , Larval settlement , Perna canaliculus
Source
Marine Biology Research, 11(6), 655-665.
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