Itaconic Acid Inhibits Growth of a Pathogenic Marine Vibrio Strain: A Metabolomics Approach

Simple item page
aut.relation.articlenumber5937en_NZ
aut.relation.issue1en_NZ
aut.relation.journalScientific Reportsen_NZ
aut.relation.volume9en_NZ
aut.researcherDrabsch, Julie
dc.contributor.authorNguyen, TVen_NZ
dc.contributor.authorAlfaro, ACen_NZ
dc.contributor.authorYoung, Ten_NZ
dc.contributor.authorGreen, Sen_NZ
dc.contributor.authorZarate, Een_NZ
dc.contributor.authorMerien, Fen_NZ
dc.date.accessioned2019-08-19T23:56:44Z
dc.date.available2019-08-19T23:56:44Z
dc.date.copyright2019en_NZ
dc.date.issued2019en_NZ
dc.description.abstractThe antimicrobial role of itaconic acid (ITA) has been recently discovered in mammalian cells. In our previous studies, we discovered that marine molluscs biosynthesise substantial quantities of ITA when exposed to marine pathogens, but its antimicrobial function to Vibrio bacteria is currently unknown. Thus, in this study, we used an untargeted gas chromatography–mass spectrometry (GC-MS) platform to identify metabolic changes of Vibrio sp. DO1 (V. corallyliticus/neptunius-like isolate) caused by ITA exposure. Vibrio sp. DO1 was cultured in Luria-Bertani broth supplemented with 3 mM sodium acetate and with different concentrations of ITA (0, 3 and 6 mM) for 24 h. The results showed that ITA completely inhibited Vibrio sp. growth at 6 mM and partially inhibited the bacterial growth at 3 mM. A principal component analysis (PCA) revealed a clear separation between metabolite profiles of Vibrio sp. DO1 in the 3 mM ITA treatment and the control, which were different in 25 metabolites. Among the altered metabolites, the accumulation of glyoxylic acid and other metabolites in glyoxylate cycle (cis-aconitic acid, isocitric acid and fumaric acid) together with the increase of isocitrate lyase (ICL) activity in the 3 mM ITA treatment compared to the control suggest that ITA inhibited Vibrio sp. growth via disruption of central carbon metabolism.en_NZ
dc.identifier.citationScientific reports, 9(1), 5937.
dc.identifier.doi10.1038/s41598-019-42315-6en_NZ
dc.identifier.issn2045-2322en_NZ
dc.identifier.issn2045-2322en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/12742
dc.publisherNature Publishing Groupen_NZ
dc.relation.urihttps://www.nature.com/articles/s41598-019-42315-6
dc.rights© 2019, The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectAntimicrobial responses; Antimicrobials; Applied immunology; Applied microbiology
dc.titleItaconic Acid Inhibits Growth of a Pathogenic Marine Vibrio Strain: A Metabolomics Approachen_NZ
dc.typeJournal Article
pubs.elements-id358689
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Applied Science
pubs.organisational-data/AUT/Health & Environmental Science/School of Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HA Science 2018 PBRF
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Nguyen_2019_Itaconic acid.pdf
Size:
1.96 MB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
AUT Grant of Licence for Tuwhera Aug 2018.pdf
Size:
276.29 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
School of Science