Metabolic Regulation of Copper Toxicity during Marine Mussel Embryogenesis

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aut.relation.issue7
aut.relation.journalMetabolites
aut.relation.startpage838
aut.relation.volume13
dc.contributor.authorYoung, Tim
dc.contributor.authorGale, Samantha L
dc.contributor.authorRagg, Norman LC
dc.contributor.authorSander, Sylvia G
dc.contributor.authorBurritt, David J
dc.contributor.authorBenedict, Billy
dc.contributor.authorLe, Dung V
dc.contributor.authorVillas-Bôas, Silas G
dc.contributor.authorAlfaro, Andrea C
dc.date.accessioned2023-08-09T22:58:21Z
dc.date.available2023-08-09T22:58:21Z
dc.date.issued2023-07-11
dc.description.abstractThe development of new tools for assessing the health of cultured shellfish larvae is crucial for aquaculture industries to develop and refine hatchery methodologies. We established a large-volume ecotoxicology/health stressor trial, exposing mussel (Perna canaliculus) embryos to copper in the presence of ethylenediaminetetraacetic acid (EDTA). GC/MS-based metabolomics was applied to identify potential biomarkers for monitoring embryonic/larval health and to characterise mechanisms of metal toxicity. Cellular viability, developmental abnormalities, larval behaviour, mortality, and a targeted analysis of proteins involved in the regulation of reactive oxygen species were simultaneously evaluated to provide a complementary framework for interpretative purposes and authenticate the metabolomics data. Trace metal analysis and speciation modelling verified EDTA as an effective copper chelator. Toxicity thresholds for P. canaliculus were low, with 10% developmental abnormalities in D-stage larvae being recorded upon exposure to 1.10 μg·L-1 bioavailable copper for 66 h. Sublethal levels of bioavailable copper (0.04 and 1.10 μg·L-1) caused coordinated fluctuations in metabolite profiles, which were dependent on development stage, treatment level, and exposure duration. Larvae appeared to successfully employ various mechanisms involving the biosynthesis of antioxidants and a restructuring of energy-related metabolism to alleviate the toxic effects of copper on cells and developing tissues. These results suggest that regulation of trace metal-induced toxicity is tightly linked with metabolism during the early ontogenic development of marine mussels. Lethal-level bioavailable copper (50.3 μg·L-1) caused severe metabolic dysregulation after 3 h of exposure, which worsened with time, substantially delayed embryonic development, induced critical oxidative damage, initiated the apoptotic pathway, and resulted in cell/organism death shortly after 18 h of exposure. Metabolite profiling is a useful approach to (1) assess the health status of marine invertebrate embryos and larvae, (2) detect early warning biomarkers for trace metal contamination, and (3) identify novel regulatory mechanisms of copper-induced toxicity.
dc.identifier.citationMetabolites, ISSN: 2218-1989 (Print); 2218-1989 (Online), MDPI AG, 13(7), 838-. doi: 10.3390/metabo13070838
dc.identifier.doi10.3390/metabo13070838
dc.identifier.issn2218-1989
dc.identifier.issn2218-1989
dc.identifier.urihttp://hdl.handle.net/10292/16529
dc.languageeng
dc.publisherMDPI AG
dc.relation.urihttps://www.mdpi.com/2218-1989/13/7/838
dc.rights.accessrightsOpenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectaquaculture
dc.subjectcopper speciation
dc.subjectecotoxicology
dc.subjectembryology
dc.subjectinvertebrate biology
dc.subjectlarval development
dc.subjectmetabolomics
dc.subjectmollusc physiology
dc.subjectoxidative stress
dc.subjectshellfish
dc.subject3205 Medical Biochemistry and Metabolomics
dc.subject3401 Analytical Chemistry
dc.subject32 Biomedical and Clinical Sciences
dc.subject34 Chemical Sciences
dc.subject14 Life Below Water
dc.subject3 Good Health and Well Being
dc.subject0301 Analytical Chemistry
dc.subject0601 Biochemistry and Cell Biology
dc.subject1103 Clinical Sciences
dc.subject3101 Biochemistry and cell biology
dc.subject3205 Medical biochemistry and metabolomics
dc.subject3401 Analytical chemistry
dc.titleMetabolic Regulation of Copper Toxicity during Marine Mussel Embryogenesis
dc.typeJournal Article
pubs.elements-id519520
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