Regulatory Effects of Mussel (Aulacomya Maoriana Iredale 1915) Larval Settlement by Neuroactive Compounds, Amino Acids and Bacterial Biofilms

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aut.relation.endpage168
aut.relation.journalAquacultureen_NZ
aut.relation.startpage158
aut.relation.volume322-323en_NZ
dark.contributor.authorAlfaro, ACen_NZ
dark.contributor.authorYoung, Ten_NZ
dark.contributor.authorGanesan, AMen_NZ
dc.date.accessioned2017-05-11T21:56:43Z
dc.date.available2017-05-11T21:56:43Z
dc.date.copyright2011en_NZ
dc.date.issued2011en_NZ
dc.description.abstractLarval settlement responses of the ribbed mussel, Aulacomya maoriana Iredale 1915, were investigated after exposure to various chemicals and mono-species bacteria. Identification of settlement inductive compounds assists in the elucidation of intermediary biochemical mechanisms involved in the neuronal control of settlement behaviour downstream from primary cue reception. Neuroactive compounds and amino acids (potassium ions, GABA, acetylcholine, l-Phenylalanine, l-Tyrosine, dopamine, epinephrine, l-Tryptophan, and 5-HTP) and planktonic bacteria, biofilms and biofilm exudates of Macrococcus sp. AMGM1, Bacillus sp. AMGB1, and Pseudoalteromonas sp. AMGP1 were tested for their abilities to induce larval settlement. Toxicity effects of each treatment also were simultaneously identified by recording larval mortalities. Results indicate that all chemicals used induced larvae to settle, with acetylcholine being the most effective (~ 24% at 10−6 M compared to < 2% in control assays). Toxicities of treatment compounds were low at optimal settlement inducing concentrations, except for l-Tryptophan (~ 32%) and GABA (~ 59%). Our data suggest that catecholamines (and their precursors) play an important role in the biochemical mechanisms of settlement for A. maoriana. While serotonin precursors did induce low levels of larval settlement at some concentrations, high toxicity responses to 5-HTP at 10−5 M, combined with complete settlement inhibition indicate that the mechanism of action may be more complex than can be elucidated in this study. Larval settlement responses to bacterial treatments were low for planktonic and biofilm phases across all three strains, and settlement inhibition was observed when larvae were exposed to biofilm exudates of all bacterial strains. Comparisons of A. maoriana responses to other endemic and worldwide distributed mussel species are provided as a means to highlight potential evolutionary differences in chemoreception mechanisms.
dc.identifier.citationAquaculture, 322, 158-168.
dc.identifier.doi10.1016/j.aquaculture.2011.08.038
dc.identifier.issn0044-8486en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/10471
dc.publisherElsevier
dc.relation.urihttp://www.sciencedirect.com.ezproxy.aut.ac.nz/science/article/pii/S0044848611006867en_NZ
dc.rightsCopyright © 2011 Elsevier Ltd. All rights reserved. This is the author’s version of a work that was accepted for publication in (see Citation). Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. The definitive version was published in (see Citation). The original publication is available at (see Publisher's Version).
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectLarval settlement; Ribbed mussels; Aulacomya maoriana; Chemical cues; Bacterial biofilms
dc.titleRegulatory Effects of Mussel (Aulacomya Maoriana Iredale 1915) Larval Settlement by Neuroactive Compounds, Amino Acids and Bacterial Biofilmsen_NZ
dc.typeJournal Article
pubs.elements-id93690
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Applied Science
pubs.organisational-data/AUT/Health & Environmental Science/School of Science
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