N-butanol Subfraction of Brassica Rapa L. Promotes Reactive Oxygen Species Production and Induces Apoptosis of A549 Lung Adenocarcinoma Cells Via Mitochondria-dependent Pathway

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aut.relation.endpage12
aut.relation.issue7en_NZ
aut.relation.journalMoleculesen_NZ
aut.relation.startpage1
aut.relation.volume23en_NZ
aut.researcherLu, Jun
dc.contributor.authorAipire, Aen_NZ
dc.contributor.authorChen, Qen_NZ
dc.contributor.authorCai, Sen_NZ
dc.contributor.authorLi, Jen_NZ
dc.contributor.authorFu, Cen_NZ
dc.contributor.authorYing, Ten_NZ
dc.contributor.authorLu, Jen_NZ
dc.contributor.authorLi, Jen_NZ
dc.date.accessioned2019-04-09T00:13:36Z
dc.date.available2019-04-09T00:13:36Z
dc.date.copyright2018en_NZ
dc.date.issued2018en_NZ
dc.description.abstractBrassica rapa L., an edible and medical vegetable, has been traditionally used in Uyghur folk medicine to treat coughs and asthma in the Xinjiang Uygur Autonomous Region, China. In this study, we prepared an n-butanol subfraction of B. rapa L. (BRBS) and investigated the anti-tumor effect on A549 lung adenocarcinoma cells. The proliferation of A549 cells was significantly inhibited by BRBS treatment in a dose- and time-dependent manner. BRBS significantly induced cell cycle arrest and apoptosis in A549 cells through increased reactive oxygen species (ROS) production and mitochondrial dysfunction characterized by a reduction in mitochondrial membrane potential and the release of cytochrome c, which promoted caspase-3 and poly(ADP-ribose) polymerase processing. Moreover, BRBS significantly suppressed the migration of A549 cells in vitro. These results suggest that BRBS inhibited A549 cell proliferation through increased ROS production and the mitochondria-dependent apoptosis pathway. Consequently, BRBS might be a potential candidate for the treatment of lung cancer.en_NZ
dc.identifier.citationMolecules, 23(7), 1687. doi:10.3390/molecules23071687
dc.identifier.doi10.3390/molecules23071687en_NZ
dc.identifier.issn1420-3049en_NZ
dc.identifier.issn1420-3049en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/12429
dc.publisherMDPI AGen_NZ
dc.relation.urihttps://www.mdpi.com/1420-3049/23/7/1687
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectBrassica rapa L.; Antitumor; Reactive oxygen species; Apoptosis; Cell cycle arrest; Migration
dc.titleN-butanol Subfraction of Brassica Rapa L. Promotes Reactive Oxygen Species Production and Induces Apoptosis of A549 Lung Adenocarcinoma Cells Via Mitochondria-dependent Pathwayen_NZ
dc.typeJournal Article
pubs.elements-id341463
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Interprofessional Health
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
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