Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

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aut.relation.issue11
aut.relation.journalInternational Journal of Molecular Sciences
aut.relation.startpage5725
aut.relation.volume25
dc.contributor.authorSlivka, EV
dc.contributor.authorShilova, NV
dc.contributor.authorObraztsova, EA
dc.contributor.authorKapustkina, DS
dc.contributor.authorKhaidukov, SV
dc.contributor.authorNokel, AY
dc.contributor.authorRyzhov, IM
dc.contributor.authorHenry, SM
dc.contributor.authorBovin, NV
dc.contributor.authorRapoport, EM
dc.date.accessioned2024-10-14T03:41:24Z
dc.date.available2024-10-14T03:41:24Z
dc.date.issued2024-05-24
dc.description.abstractGlycans of MVs are proposed to be candidates for mediating targeting specificity or at least promoting it. In contrast to exosomes, glycomic studies of MVs are largely absent. We studied the glycoprofile of endothelial cell-derived MVs using 21 plant lectins, and the results show the dominance of oligolactosamines and their α2-6-sialylated forms as N-glycans and low levels of α2-3-sialylated glycans. The low levels of α2-3-sialosides could not be explained by the action of extracellular glycosidases. Additionally, the level of some Man-containing glycans was also decreased in MVs. Spatial masking as the causative relationship between these low level glycans (as glycosphingolipids) by integral proteins or proteoglycans (thus, their lack of interaction with lectins) seems unlikely. The results suggest that integral proteins do not pass randomly into MVs, but instead only some types, differing in terms of their specific glycosylation, are integrated into MVs.
dc.identifier.citationInternational Journal of Molecular Sciences, ISSN: 1661-6596 (Print); 1422-0067 (Online), MDPI AG, 25(11), 5725-. doi: 10.3390/ijms25115725
dc.identifier.doi10.3390/ijms25115725
dc.identifier.issn1661-6596
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10292/18127
dc.languageeng
dc.publisherMDPI AG
dc.relation.urihttps://www.mdpi.com/1422-0067/25/11/5725
dc.rights© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
dc.rights.accessrightsOpenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectendothelial cells
dc.subjectextracellular vesicles
dc.subjectglycans
dc.subjectglycosphingolipids
dc.subjectmicrovesicles
dc.subject3101 Biochemistry and Cell Biology
dc.subject31 Biological Sciences
dc.subject0399 Other Chemical Sciences
dc.subject0604 Genetics
dc.subject0699 Other Biological Sciences
dc.subjectChemical Physics
dc.subject3101 Biochemistry and cell biology
dc.subject3107 Microbiology
dc.subject3404 Medicinal and biomolecular chemistry
dc.subject.meshPolysaccharides
dc.subject.meshPlant Lectins
dc.subject.meshHumans
dc.subject.meshEndothelial Cells
dc.subject.meshGlycosylation
dc.subject.meshCell-Derived Microparticles
dc.subject.meshEndothelial Cells
dc.subject.meshHumans
dc.subject.meshPolysaccharides
dc.subject.meshPlant Lectins
dc.subject.meshGlycosylation
dc.subject.meshCell-Derived Microparticles
dc.subject.meshPolysaccharides
dc.subject.meshPlant Lectins
dc.subject.meshHumans
dc.subject.meshEndothelial Cells
dc.subject.meshGlycosylation
dc.subject.meshCell-Derived Microparticles
dc.titleSurface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins
dc.typeJournal Article
pubs.elements-id569716
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