Antibacterial Activity of Selected Fractions Extract of Coptis chinensis Franch Rhizomes

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aut.relation.endpage990
aut.relation.issue3en_NZ
aut.relation.journalMedico-Legal Updateen_NZ
aut.relation.startpage985
aut.relation.volume20en_NZ
aut.researcherAlshaikhli, Hisham
dc.contributor.authorAlshaikhli, Hen_NZ
dc.date.accessioned2020-08-30T23:01:11Z
dc.date.available2020-08-30T23:01:11Z
dc.date.copyright2020-07-24en_NZ
dc.date.issued2020-07-24en_NZ
dc.description.abstractThe antibacterial activity of different fractions of selected extract of Coptis chinensis Franch rhizome was evaluated on gram positive bacteria. Three extracts were tested at concentrations ranging from 1 mg/ml, 10 mg/ml, and 100 mg/ml in vitro on three bacterial strains, Staphylococcus aureus, Streptococcus pneumoniae, and Bacillus anthracis, which was done by performing disc diffusion for growth inhibition. The methanol extract at concentrations of 100 mg/ml displayed the most significant zone of inhibition of (22.67 ± 3.055mm), (21.67 ± 3.512 mm), (16.67 ± 1.528 mm) against Streptococcus pneumoniae, Staphylococcus aureus, and Bacillus anthracis, respectively. The TLC separation of the extracts showed that each of them may contains constituents that are phenolic in nature. From the Rf values of the spots found in the various extracts is that the Coptis chinensis Franch rhizome contains some other non-phenolic compounds responsible for its effect. The selected methanol extract is then subjected to column chromatography and collected 4 fractions. Out of the four fractions, F2 fraction demonstrates the most significant zone of inhibition of 15.00 ± 1.000 mm, 12.33 ± 1.528 mm and 10.00 ± 2.000 mm against Streptococcus pneumoniae, Staphylococcus aureus, and Bacillus anthracis, respectively.
dc.identifier.citationMedico Legal Update, 20(3), 985-990. https://doi.org/10.37506/mlu.v20i3.1531
dc.identifier.doi10.37506/mlu.v20i3.1531en_NZ
dc.identifier.issn0971-720Xen_NZ
dc.identifier.urihttps://hdl.handle.net/10292/13615
dc.publisherWorld Informations Syndicateen_NZ
dc.relation.urihttp://ijop.net/index.php/mlu/article/view/1531
dc.rightsArticles in Medico-legal Update journal are Open Access articles published under the Creative Commons CC BY License Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ . This license permits use, distribution and reproduction in any medium, provided the original work and source is properly cited.
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectCoptis chinensis; Fractions; Antibacterial activity; Zone of inhibition
dc.titleAntibacterial Activity of Selected Fractions Extract of Coptis chinensis Franch Rhizomesen_NZ
dc.typeJournal Article
pubs.elements-id390590
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
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