Effect of Bone Marrow Mesenchymal Stem Cells-derived Exosomes on Diabetes-induced Retinal Injury: Implication of Wnt/ B-catenin Signaling Pathway

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aut.relation.articlenumber113554en_NZ
aut.relation.endpage113554
aut.relation.journalBiomedicine & Pharmacotherapyen_NZ
aut.relation.startpage113554
aut.relation.volume154en_NZ
aut.researcherHassouna, Amira
dc.contributor.authorEbrahim, Nen_NZ
dc.contributor.authorEl-Halim, HEAen_NZ
dc.contributor.authorHelal, OKen_NZ
dc.contributor.authorEl-Azab, NE-Een_NZ
dc.contributor.authorBadr, OAMen_NZ
dc.contributor.authorHassouna, Aen_NZ
dc.contributor.authorSaihati, HAAen_NZ
dc.contributor.authorAborayah, NHen_NZ
dc.contributor.authorEmam, HTen_NZ
dc.contributor.authorEl-wakeel, HSen_NZ
dc.contributor.authorAljasir, Men_NZ
dc.contributor.authorEl-Sherbiny, Men_NZ
dc.contributor.authorSarg, NASen_NZ
dc.contributor.authorShaker, GAen_NZ
dc.contributor.authorMostafa, Oen_NZ
dc.contributor.authorSabry, Den_NZ
dc.contributor.authorFouly, MAKen_NZ
dc.contributor.authorForsyth, NRen_NZ
dc.contributor.authorElsherbiny, NMen_NZ
dc.contributor.authorSalim, RFen_NZ
dc.date.accessioned2022-09-26T22:26:51Z
dc.date.available2022-09-26T22:26:51Z
dc.date.copyright2022-10en_NZ
dc.date.issued2022-10en_NZ
dc.description.abstractBackground Diabetic retinopathy (DR) is a serious microvascular complication of diabetes mellitus. Mesenchymal stem cells are currently studied as therapeutic strategy for management of DR. Exosomes, considered as a promising cell-free therapy option, display biological functions similar to those of their parent cells. In retinal development, Wnt/b-catenin signaling provides key cues for functional progression. The present study aimed to evaluate the potential efficacy of bone marrow-derived mesenchymal stem cell-derived exosomes (BM-MSCs-Ex) in diabetes-induced retinal injury via modulation of the Wnt/ b-catenin signaling pathway. Methods Eighty-one rats were allocated into 6 groups (control, DR, DR + DKK1, DR + exosomes, DR + Wnt3a and DR + exosomes+Wnt3a). Evaluation of each group was via histopathological examination, assessment of gene and/or protein expression concerned with oxidative stress (SOD1, SOD2, Nox2, Nox4, iNOS), inflammation (TNF-α, ICAM-1, NF-κB) and angiogenesis (VEGF, VE-cadherin). Results Results demonstrated that exosomes blocked the wnt/b-catenin pathway in diabetic retina concomitant with significant reduction of features of DR as shown by downregulation of retinal oxidants, upregulation of antioxidant enzymes, suppression of retinal inflammatory and angiogenic markers. These results were further confirmed by histopathological results, fundus examination and optical coherence tomography. Additionally, exosomes ameliorative effects abrogated wnt3a-triggered retinal injury in DR. Conclusion Collectively, these data demonstrated that exosomes ameliorated diabetes-induced retinal injury via suppressing Wnt/ b-catenin signaling with subsequent reduction of oxidative stress, inflammation and angiogenesis.
dc.identifier.citationBiomedicine & Pharmacotherapy, Volume 154, October 2022, 113554.
dc.identifier.doi10.1016/j.biopha.2022.113554en_NZ
dc.identifier.issn0753-3322en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/15475
dc.languageenen_NZ
dc.publisherElsevier BVen_NZ
dc.relation.urihttp://dx.doi.org/10.1016/j.biopha.2022.113554en_NZ
dc.rights© 2022 The Author(s). Published by Elsevier Masson SAS. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectDiabetic retinopathy; Mesenchymal stem cells; Exosomes
dc.titleEffect of Bone Marrow Mesenchymal Stem Cells-derived Exosomes on Diabetes-induced Retinal Injury: Implication of Wnt/ B-catenin Signaling Pathwayen_NZ
dc.typeJournal Article
pubs.elements-id478463
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Faculty of Health & Environmental Science
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Public Health & Interdisciplinary Studies
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Public Health & Interdisciplinary Studies/Biosciences Department
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
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