Effect of Overexpression of LPAAT and GPD1 on Lipid Synthesis and Composition in Green Microalga Chlamydomonas Reinhardtii

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aut.relation.endpage1719
aut.relation.issue3en_NZ
aut.relation.journalJournal of Applied Phycologyen_NZ
aut.relation.startpage1711
aut.relation.volume30en_NZ
aut.researcherLu, Jun
dc.contributor.authorWang, Cen_NZ
dc.contributor.authorLi, Yen_NZ
dc.contributor.authorLu, Jen_NZ
dc.contributor.authorDeng, Xen_NZ
dc.contributor.authorLi, Hen_NZ
dc.contributor.authorHu, Zen_NZ
dc.date.accessioned2020-06-28T22:48:39Z
dc.date.available2020-06-28T22:48:39Z
dc.date.copyright2018en_NZ
dc.date.issued2018en_NZ
dc.description.abstractBiodiesel is an alternative energy source which has attracted increasing attention lately. Although algae-based biodiesel production has many benefits, it is still far from industrial application. Research suggests that improving lipid quality and production through genetic engineering of metabolic pathways will be the most promising way. To enhance lipid content, both lysophosphatidic acyltransferase gene (c-lpaat) and glycerol-3-phosphate dehydrogenase gene (c-gpd1), optimized according to the codon bias of Chlamydomonas reinhardtii, were inserted into the genomic DNA of model microalga C. reinhardtii by the glass bead method. Transgenic algae were screened by zeomycin resistance and RT-PCR. The transcription levels of inserted genes and the fatty acid content were significantly increased after intermittent heat shock. Most of all, the transcription levels of c-lpaat and c-gpd1 were increased 5.3 and 8.6 times after triple heat shocks, resulting in an increase of 44.5 and 67.5% lipid content, respectively. Furthermore, the content of long-chain saturated fatty acids and monounsaturated fatty acids, especially C18 and C18:1t, notably increased, while unsaturated fatty acids dramatically decreased. The results of this study offer a new strategy combining genetic manipulation and intermittent heat shock to enhance lipid production, especially the production of long-chain saturated fatty acids, using C. reinhardtii.en_NZ
dc.identifier.citationJournal of Applied Phycology, 30, 1711–1719 (2018). https://doi.org/10.1007/s10811-017-1349-2
dc.identifier.doi10.1007/s10811-017-1349-2en_NZ
dc.identifier.issn0921-8971en_NZ
dc.identifier.issn1573-5176en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/13458
dc.publisherSpringer Nature
dc.relation.urihttps://link.springer.com/article/10.1007%2Fs10811-017-1349-2
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectChlamydomonas reinhardtii; Lipid content; Triacylglycerol synthesis; Transgenic algae; Intermittent heat shock
dc.titleEffect of Overexpression of LPAAT and GPD1 on Lipid Synthesis and Composition in Green Microalga Chlamydomonas Reinhardtiien_NZ
dc.typeJournal Article
pubs.elements-id322876
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Interprofessional Health
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
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