Ultra-fast Glyco-coating of Non-biological Surfaces

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aut.relation.issue1en_NZ
aut.relation.journalInternational Journal of Molecular Sciencesen_NZ
aut.relation.volume17en_NZ
dc.contributor.authorWilliams, Een_NZ
dc.contributor.authorBarr, Ken_NZ
dc.contributor.authorKorchagina, Een_NZ
dc.contributor.authorTuzikov, Aen_NZ
dc.contributor.authorHenry, Sen_NZ
dc.contributor.authorBovin, Nen_NZ
dc.date.accessioned2016-10-24T22:14:24Z
dc.date.available2016-10-24T22:14:24Z
dc.date.copyright2016-01-16en_NZ
dc.date.issued2016-01-16en_NZ
dc.description.abstractThe ability to glycosylate surfaces has medical and diagnostic applications, but there is no technology currently recognized as being able to coat any surface without the need for prior chemical modification of the surface. Recently, a family of constructs called function-spacer-lipids (FSL) has been used to glycosylate cells. Because it is known that lipid-based material can adsorb onto surfaces, we explored the potential and performance of cell-labelling FSL constructs to “glycosylate” non-biological surfaces. Using blood group A antigen as an indicator, the performance of a several variations of FSL constructs to modify a large variety of non-biological surfaces was evaluated. It was found the FSL constructs when optimised could in a few seconds glycosylate almost any non-biological surface including metals, glass, plastics, rubbers and other polymers. Although the FSL glycan coating was non-covalent, and therefore temporary, it was sufficiently robust with appropriate selection of spacer and surface that it could capture anti-glycan antibodies, immobilize cells (via antibody), and withstand incubation in serum and extensive buffer washing, making it suitable for diagnostic and research applications.en_NZ
dc.identifier.citationInternational Journal of Molecular Sciences. 2016, 17(1), 118; doi:10.3390/ijms17010118
dc.identifier.doi10.3390/ijms17010118en_NZ
dc.identifier.issn1661-6596en_NZ
dc.identifier.issn1422-0067en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/10093
dc.languageengen_NZ
dc.publisherMDPI AGen_NZ
dc.relation.urihttp://dx.doi.org/10.3390/ijms17010118
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectBlood group Aen_NZ
dc.subjectFunction-spacer-lipiden_NZ
dc.subjectGlyco-coatingen_NZ
dc.subjectGlyco-landscapeen_NZ
dc.subjectNanofibresen_NZ
dc.subjectShear stressen_NZ
dc.subjectSurface-coatingen_NZ
dc.titleUltra-fast Glyco-coating of Non-biological Surfacesen_NZ
dc.typeJournal Article
pubs.elements-id196901
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Design & Creative Technologies
pubs.organisational-data/AUT/Design & Creative Technologies/Engineering, Computer & Mathematical Sciences
pubs.organisational-data/AUT/Design & Creative Technologies/Faculty Central
pubs.organisational-data/AUT/Design & Creative Technologies/School of Engineering
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