Next-generation Sequencing As an Advanced Supplementary Tool for the Diagnosis of Pathogens in Lower Respiratory Tract Infections: An Observational Trial in Xi'an, China

Simple item page
aut.relation.articlenumber14en_NZ
aut.relation.issue2en_NZ
aut.relation.journalBiomedical Reportsen_NZ
aut.relation.volume16en_NZ
aut.researcherDrabsch, Julie
dc.contributor.authorShao, Jen_NZ
dc.contributor.authorHassouna, Aen_NZ
dc.contributor.authorWang, Yen_NZ
dc.contributor.authorZhang, Ren_NZ
dc.contributor.authorZhen, Len_NZ
dc.contributor.authorLi, Ren_NZ
dc.contributor.authorChen, Men_NZ
dc.contributor.authorLiu, Cen_NZ
dc.contributor.authorWang, Xen_NZ
dc.contributor.authorZhang, Men_NZ
dc.contributor.authorWang, Pen_NZ
dc.contributor.authorYuan, Sen_NZ
dc.contributor.authorChen, Jen_NZ
dc.contributor.authorLu, Jen_NZ
dc.date.accessioned2022-08-03T02:17:27Z
dc.date.available2022-08-03T02:17:27Z
dc.date.copyright2022en_NZ
dc.date.issued2022en_NZ
dc.description.abstractThe application of next-generation sequencing (NGS) in routine clinical analysis is still limited. The significance of NGS in the identification of pathogens of lower respiratory tract infection should be assessed as part of routine clinical bacterial examinations and chest imaging results. In the present study, the alveolar lavage fluid samples of 30 patients (25 males and 5 females, aged 19-92 years old, with a median age of 62) were examined by routine bacterial culture and NGS, and the results of pathogen detection and identification were compared. Chest imaging showed consoli-dation in all 30 patients (100%), and pleural effusion in 13 of the 30 patients (43.33%). The routine bacterial culture of the lavage solution was only positive in 14 of the 30 patients (46.6%), and negative in 16 patients (53.33%). However, the positive rate of NGS test results of the lavage fluid was 100%. A total of 12 cases (40%) were completely consistent with the routine bacterial culture test, with 56 other pathogens of mixed infection detected, accounting for the short comings of the routine bacterial examination. Although NGS cannot distinguish between live and dead bacteria, it is still a useful detection technology for accurate diagnosis of clinical infectious diseases. It is worthy of adaptation in the clinic for more effective clinical management and treatment of the lower respiratory airway infection in addition to the routine bacterial culture testing.en_NZ
dc.identifier.citationBiomedical Reports, 16, 14. https://doi.org/10.3892/br.2021.1497
dc.identifier.doi10.3892/br.2021.1497en_NZ
dc.identifier.issn2049-9434en_NZ
dc.identifier.issn2049-9442en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/15337
dc.publisherSpandidos Publications
dc.relation.urihttps://www.spandidos-publications.com/10.3892/br.2021.1497
dc.rights© Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectPulmonary infection; Diagnosis; Next‑generation sequencing; Bronchoalveolar lavage fluid; Bacterial culture
dc.titleNext-generation Sequencing As an Advanced Supplementary Tool for the Diagnosis of Pathogens in Lower Respiratory Tract Infections: An Observational Trial in Xi'an, Chinaen_NZ
dc.typeJournal Article
pubs.elements-id447984
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Faculty of Health & Environmental Science
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Public Health & Interdisciplinary Studies
pubs.organisational-data/AUT/Faculty of Health & Environmental Science/School of Public Health & Interdisciplinary Studies/Biosciences Department
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HI Interprofessional 2018 PBRF
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Shao et al._2022_Next generation sequencing.pdf
Size:
305.98 KB
Format:
Adobe Portable Document Format
Description:
Journal article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
AUT Grant of Licence for Tuwhera Jun 2021.pdf
Size:
360.95 KB
Format:
Adobe Portable Document Format
Description:
Download
Collections
School of Public Health and Interdisciplinary Studies