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dc.identifier.citationHeliyon, 6(7), e04357.
dc.description.abstractMolluscan extracts confer a wide range of health promoting properties, one of them is cytotoxicity. Extraction and processing can affect the efficacy and properties of bioactive molecules. New Zealand (NZ) surf clams have never been thoroughly studied for bioactives until recently. However, the effect of cold and heat extraction procedure on biochemical composition and cytotoxic activities of NZ surf clam remains unanswered. The objective is to compare the effects on cytotoxicity of three NZ surf clams (Diamond shell, Crassula aequilatera; Storm shell, Mactra murchisoni; and Deepwater Tua tua, Paphies donacina) extracts via cold or heat process across cancer cell lines to find out which process can preserve bioactivity better. Fractions of extracts prepared via cold or heat procedures were tested for cell growth inhibition, apoptosis induction and cell cycle arrest in seven cancer cell lines. Apoptosis was induced through all cell lines, as further evidenced in Caspase-3/7 activities. Cell cycle arrest was focused on G2/M- and S- phases. Petroleum ether and ethyl acetate fractions, with the greatest bioactivity in this study, are rich in lipids and proteins, indicating likely bioactive sources. Cold preparation was responsible for the lowest cancer cell viability and induced greater apoptosis. Cold process retained better bioactivity/cytotoxicity than that of heat-processed extracts. This information may guide future health/nutraceutical clam product development.en_NZ
dc.rights© 2020 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (
dc.subjectFood science; Food technology; Surf clam; Drying; Cytotoxicity; Storm shell; Tua tua; Dimond shell; Crassula aequilatera; Mactra murchisoni; Paphies donacina; New Zealand
dc.titleEffects of Preparation Method on the Biochemical Characterization and Cytotoxic Activity of New Zealand Surf Clam Extractsen_NZ
dc.typeJournal Article
dark.contributor.authorOdeleye, Ten_NZ
dark.contributor.authorZeng, Zen_NZ
dark.contributor.authorWhite, WLen_NZ
dark.contributor.authorWang, KSen_NZ
dark.contributor.authorLi, Hen_NZ
dark.contributor.authorXu, Xen_NZ
dark.contributor.authorXu, Hen_NZ
dark.contributor.authorLi, Jen_NZ
dark.contributor.authorYing, Ten_NZ
dark.contributor.authorZhang, Ben_NZ
dark.contributor.authorFeng, Ten_NZ
dark.contributor.authorLu, Jen_NZ

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