Use of Membrane Technology for Concentration of Bioactive Peptides from Geoduck (Panopea zelandica)

aut.embargoNoen_NZ
aut.filerelease.date2020-10-12
aut.thirdpc.containsNoen_NZ
aut.thirdpc.permissionNoen_NZ
aut.thirdpc.removedNoen_NZ
dc.contributor.advisorWhite, Lindsey
dc.contributor.advisorHofman, Kathleen
dc.contributor.authorBettjeman, Bodhi
dc.date.accessioned2017-10-12T02:58:42Z
dc.date.available2017-10-12T02:58:42Z
dc.date.copyright2017
dc.date.created2017
dc.date.issued2017
dc.date.updated2017-10-11T01:20:35Z
dc.description.abstractEnzymatic digestion of proteins generates biologically active peptides which can be concentrated by filtration with the appropriate membranes. This work uses a hydrolysate derived from the New Zealand geoduck Panopea zelandica and compares the effectiveness of membranes with varying molecular weight cut-offs (MWCOs) and surface charges for carrying out selective separations of the peptides. A membrane with a MWCO of 2.5 kDa was found to be most effective for obtaining the greatest differences between the permeate and retentate from the membranes when separating by size alone. For separating peptides based on charge the choice of membrane and pH was important. The results obtained suggested that each hydrolysate has different optimum conditions for achieving separation. A challenge when analysing the effects of membranes on peptide mixtures is quantifying the changes that have occurred. The work presented here introduced a multivariate approach to interpretation of complete sets of HPLC data that had not previously been used for analysis of complex peptide mixtures. This approach was used to measure the effectiveness of the membrane separations, and allowed comparisons to be drawn between these separations and the peptide bioactivity as determined using the FRAP assay.en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/10862
dc.language.isoenen_NZ
dc.publisherAuckland University of Technology
dc.rights.accessrightsOpenAccess
dc.subjectMembraneen_NZ
dc.subjectBioactiveen_NZ
dc.subjectPeptideen_NZ
dc.subjectHPLCen_NZ
dc.subjectMultivariate analysisen_NZ
dc.subjectGeoducken_NZ
dc.subjectAntioxidanten_NZ
dc.titleUse of Membrane Technology for Concentration of Bioactive Peptides from Geoduck (Panopea zelandica)en_NZ
dc.typeThesis
thesis.degree.grantorAuckland University of Technology
thesis.degree.levelMasters Theses
thesis.degree.nameMaster of Scienceen_NZ
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