Influence of Operating Conditions on Reuterin Production by Resting Cells of Lactobacillus Reuteri DPC16

aut.embargoNoen_NZ
aut.thirdpc.containsNoen_NZ
dc.contributor.advisorGutierrez-Maddox, Noemi
dc.contributor.advisorMutukumira, Anthony N
dc.contributor.authorSun, Yuanze
dc.date.accessioned2020-10-19T00:04:16Z
dc.date.available2020-10-19T00:04:16Z
dc.date.copyright2020
dc.date.issued2020
dc.date.updated2020-10-18T20:45:35Z
dc.description.abstractReuterin (3 hydroxypropionaldehyde), secreted from L. reuteri strains, is a potentially valuable chemical and broad spectrum antimicrobial substance. The patented bacteria strain, L. reuteri DPC16, is supported by Drapac Ltd, and is successfully used in commerce. However, the low yield of reuterin greatly restricts its commercial use. To improve the conversion of glycerol to reuterin, this project studied the two step process of reuterin production from glycerol to reuterin by L. reuteri DPC16. The influence of initial glycerol concentration, biomass concentration, pH value, culture age, conversion time and temperature on the production of reuterin were investigated. The results showed that maximum reuterin production was achieved by fermenting 350mmol/L (initial concentration) of glycerol for 2h at 25 and pH 6.8 using 25g/L of 20h old resting DPC16 cells.en_NZ
dc.identifier.urihttps://hdl.handle.net/10292/13721
dc.language.isoenen_NZ
dc.publisherAuckland University of Technology
dc.rights.accessrightsOpenAccess
dc.subjectLactobacillus reuteri DPC16en_NZ
dc.subjectReuterinen_NZ
dc.subject3 hydroxypropionaldehydeen_NZ
dc.subjectBioconversionen_NZ
dc.titleInfluence of Operating Conditions on Reuterin Production by Resting Cells of Lactobacillus Reuteri DPC16en_NZ
dc.typeThesisen_NZ
thesis.degree.grantorAuckland University of Technology
thesis.degree.levelMasters Theses
thesis.degree.nameMaster of Scienceen_NZ
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