Rothschild, Jeffrey ADudley-Rode, HarrisonCarpenter, HarrietSmith, AbbiePlews, Daniel JMaunder, Ed2026-04-072026-04-072025-12-09Journal of Applied Physiology, ISSN: 8750-7587 (Print); 1522-1601 (Online), American Physiological Society, 140(1), 76-87. doi: 10.1152/japplphysiol.00861.20258750-75871522-1601http://hdl.handle.net/10292/20875Background: While some studies report attenuated net muscle glycogenolysis with carbohydrate ingestion, others show no effect, possibly due to small sample sizes or methodological differences. Objective: To determine whether carbohydrate ingestion during endurance exercise reduces net skeletal muscle glycogen use, and to identify potential moderating factors. Methods: A meta-analysis was conducted using data from 31 studies which included 48 unique effect sizes derived from crossover trials comparing carbohydrate vs. placebo ingestion during prolonged endurance exercise. Standardized mean differences (SMDs) in net muscle glycogen utilization were calculated. A multilevel random-effects model accounted for repeated estimates within studies. Subgroup and meta-regression analyses tested potential moderators. Sensitivity analyses were conducted using a range of plausible pre/post correlation values. Results: Carbohydrate ingestion was associated with a small but statistically significant muscle glycogen-sparing effect (SMD = –0.16, 95% CI: –0.30 to –0.02, p = 0.021). Subgroup and moderator analyses revealed no significant effects of exercise mode, carbohydrate type, ingestion rate, or pre-exercise glycogen on the observed effect. Translating the standardized effect into absolute units, carbohydrate ingestion was estimated to spare ~24 mmol kg-1 dry weight (95% CI: 4 to 45 mmol kg -1) of muscle glycogen, relative to placebo, during ~100 min of exercise. Conclusion: Carbohydrate ingestion during endurance exercise leads to a small but statistically significant reduction in net skeletal muscle glycogen utilization. Although no consistent moderating variables were identified, the direction of effect was consistent across studies, and the absolute magnitude of sparing may be physiologically meaningful during prolonged or repeated efforts.Copyright © 2026 The Authors. Licensed under Creative Commons Attribution CC-BY-NC-ND 4.0. Published by the American Physiological Society.exerciseglycogenolysismetabolismnutritionskeletal muscle42 Health Sciences4207 Sports Science and ExercisePreventionPhysical ActivityBehavioral and Social ScienceClinical ResearchClinical Trials and Supportive Activities06 Biological Sciences11 Medical and Health SciencesPhysiology31 Biological sciences32 Biomedical and clinical sciences42 Health sciencesHumansMuscle, SkeletalGlycogenDietary CarbohydratesExercisePhysical EnduranceMuscle, SkeletalHumansGlycogenDietary CarbohydratesExercisePhysical EnduranceHumansMuscle, SkeletalGlycogenDietary CarbohydratesExercisePhysical EnduranceJournal ArticleOpenAccess10.1152/japplphysiol.00861.2025