Hunter, BenMaunder, EdJones, Andrew MGallo, GabrieleMuniz-Pumares, Daniel2025-12-082025-12-082025-03-27Experimental Physiology, ISSN: 0958-0670 (Print); 1469-445X (Online), Wiley, 110(11), 1612-1624. doi: 10.1113/EP0921200958-06701469-445Xhttp://hdl.handle.net/10292/20351Endurance athletes routinely complete physiological assessments to predict performance, inform training programmes and monitor subsequent training adaptations. This profiling is typically performed with the athlete in a 'fresh' (i.e., rested) condition, but physiological profiling variables deteriorate during prolonged exercise. Durability has been defined as the resilience to the deterioration of physiological variables and performance during or following prolonged exercise. Herein, we review the current approaches to measure durability. The construction of the fatiguing protocol affects durability profiles, with greater relative intensity and duration resulting in more marked deterioration of baseline measures. The design of durability assessments should control for factors that could impact durability measurements, such as nutrition and environmental characteristics, to ensure that outcomes are repeatable and can be compared between athletes or over time in the same athlete. The selection of these parameters should be based on the proposed research question or applied context and take account of the training status of the athlete. Accordingly, this review highlights important considerations to ensure that protocols for profiling durability in research and applied practice are appropriate.© 2025 The Author(s). Experimental Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/endurance performanceexercise capacityfatigue resistanceendurance performanceexercise capacityfatigue resistance42 Health Sciences4207 Sports Science and ExercisePhysical ActivityClinical Research2.1 Biological and endogenous factorsGeneric health relevance0606 Physiology1106 Human Movement and Sports Sciences1116 Medical PhysiologyPhysiology3109 Zoology3208 Medical physiology4207 Sports science and exerciseHumansPhysical EnduranceExerciseAthletic PerformanceAthletesAdaptation, PhysiologicalMuscle FatigueHumansExerciseAdaptation, PhysiologicalMuscle FatiguePhysical EnduranceAthletic PerformanceAthletesHumansPhysical EnduranceExerciseAthletic PerformanceAthletesAdaptation, PhysiologicalMuscle FatigueJournal ArticleOpenAccess10.1113/EP092120