Slivka, EVShilova, NVObraztsova, EAKapustkina, DSKhaidukov, SVNokel, AYRyzhov, IMHenry, SMBovin, NVRapoport, EM2024-10-142024-10-142024-05-24International Journal of Molecular Sciences, ISSN: 1661-6596 (Print); 1422-0067 (Online), MDPI AG, 25(11), 5725-. doi: 10.3390/ijms251157251661-65961422-0067http://hdl.handle.net/10292/18127Glycans of MVs are proposed to be candidates for mediating targeting specificity or at least promoting it. In contrast to exosomes, glycomic studies of MVs are largely absent. We studied the glycoprofile of endothelial cell-derived MVs using 21 plant lectins, and the results show the dominance of oligolactosamines and their α2-6-sialylated forms as N-glycans and low levels of α2-3-sialylated glycans. The low levels of α2-3-sialosides could not be explained by the action of extracellular glycosidases. Additionally, the level of some Man-containing glycans was also decreased in MVs. Spatial masking as the causative relationship between these low level glycans (as glycosphingolipids) by integral proteins or proteoglycans (thus, their lack of interaction with lectins) seems unlikely. The results suggest that integral proteins do not pass randomly into MVs, but instead only some types, differing in terms of their specific glycosylation, are integrated into MVs.© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).https://creativecommons.org/licenses/by/4.0/endothelial cellsextracellular vesiclesglycansglycosphingolipidsmicrovesicles3101 Biochemistry and Cell Biology31 Biological Sciences0399 Other Chemical Sciences0604 Genetics0699 Other Biological SciencesChemical Physics3101 Biochemistry and cell biology3107 Microbiology3404 Medicinal and biomolecular chemistryPolysaccharidesPlant LectinsHumansEndothelial CellsGlycosylationCell-Derived MicroparticlesEndothelial CellsHumansPolysaccharidesPlant LectinsGlycosylationCell-Derived MicroparticlesPolysaccharidesPlant LectinsHumansEndothelial CellsGlycosylationCell-Derived MicroparticlesJournal ArticleOpenAccess10.3390/ijms25115725