Sabadel, Amandine JMRiekenberg, PhilipAyala-Diaz, MonicaBelk, Mark CBennett, JerushaBode, AntonioBury, Sarah JDabouineau, LaurentDelgado, JosetteFinucci, BrittanyGarcía-Seoane, RitaGiari, LuisaHenkens, JessicaIJsseldijk, Lonneke LJoling, TijsKerr-Hislop, OllieMacLeod, Colin DMeyer, LaurenMcGill, Rona ARNegro, EleonoraQuillfeldt, PetraReed, CecileRoberts, ChloeSayyaf Dezfuli, BahramSchmidt, OlafSturbois, AnthonySuchomel, Andrew DThieltges, David Wvan der Lingen, Carl Dvan der Meer, Marcel TJViana, Inés GWeston, MarkWillis, Trevor JFilion, Antoine2025-05-012025-05-012025-04-14Sci Data, ISSN: 2052-4463 (Print); 2052-4463 (Online), Springer Science and Business Media LLC, 12(1), 623-. doi: 10.1038/s41597-025-04970-52052-44632052-4463http://hdl.handle.net/10292/19139Over the past decades, stable isotopes have been infrequently used to characterise host-parasite trophic relationships. This is because we have not yet identified consistent patterns in stable isotope values between parasites and their host tissues across species, which are crucial for understanding host-parasite dynamics. To address this, we initiated a worldwide collaboration to establish a unique database of stable isotope values of novel host-parasite pairs, effectively doubling the existing data in published literature. This database includes nitrogen, carbon, and sulphur stable isotope values. We present 3213 stable isotope data entries, representing 586 previously unpublished host-parasite pairs. Additionally, while existing literature was particularly limited in sulphur isotope values, we tripled information on this crucial element. By publishing unreported host-parasite pairs from previously unsampled areas of the world and using appropriate host tissues, our dataset stands unparalleled. We anticipate that end-users will utilise our database to uncover generalisable patterns, deepening our understanding of the complexities of parasite-host relationships and driving future research efforts in stable isotope parasitology.Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/4101 Climate Change Impacts and Adaptation41 Environmental Sciences2.2 Factors relating to the physical environment2.1 Biological and endogenous factorsInfectionHost-Parasite InteractionsAnimalsNitrogen IsotopesCarbon IsotopesDatabases, FactualSulfur IsotopesParasitesAnimalsParasitesCarbon IsotopesNitrogen IsotopesSulfur IsotopesDatabases, FactualHost-Parasite InteractionsHost-Parasite InteractionsAnimalsNitrogen IsotopesCarbon IsotopesDatabases, FactualSulfur IsotopesParasitesJournal ArticleOpenAccess10.1038/s41597-025-04970-5