2026-09-22T18:25:50Zhttps://openrepository.aut.ac.nz/server/oai/requestoai:openrepository.aut.ac.nz:10292/110282026-06-13T16:24:19Zcom_10292_3col_10292_5
AUT Research Repository
advisor
Henry, Stephen
author
Velamoor, Sailakshmi
2017-11-26T21:04:05Z
2017-11-26T21:04:05Z
2017
https://hdl.handle.net/10292/11028
In recent years, culturing cells on synthetic surfaces has a growing importance in many fields ranging from simple two-dimensional cell culture to biomedical applications such as tissue engineering, regenerative medicine, medical devices and biosensor chips. The extent of cell adhesion to these synthetic surfaces or biomaterials plays a decisive role in regulating the cells’ subsequent proliferation and differentiation. While a wide range of surface modification technologies are available to modify surfaces and promote cell adhesion, many are either complicated or expensive. Kode™ Technology FSL constructs have the ability to modify both cells and surfaces, and thus have the potential to be adapted to cellular adhesion.
The aim of this study was to adhere proliferating non-adherent Jurkat cells to inert surfaces using Kode™ Technology. This was investigated using a biotin-streptavidin model. Findings reveal ability of FSL constructs to modify both cells and surfaces and facilitate direct adhesion in a dose dependent manner. Some cytotoxicity was observed under high insertion concentration. Although, presence of serum severely inhibits FSL insertion and retention, and cell adhesion to FSL modified surfaces, Kode™ has the potential to facilitate cellular adhesion to plastic and metal surfaces.
en
FSL- biotin
Non- adherent cells
Surface modification
Kode
Enhancing Attachment of Non-Adherent Cells on Inert Surfaces
Thesis
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URL
https://openrepository.aut.ac.nz/bitstreams/256d588a-fd20-4e68-a0f8-c0e219f99d8f/download
File
MD5
a9420e544f3ef2bee732974b1b1422bf
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application/pdf
VelamoorS.pdf
URL
https://openrepository.aut.ac.nz/bitstreams/cd336f00-24f3-4ceb-9d46-8df0b4941416/download
File
MD5
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text/plain
VelamoorS.pdf.txt