Antioxidant and Cytotoxicity Activities of Butylated Hydroxytoluene Ligands Capped Gold Nanoparticles

Ahmad, MH
Yehye, WA
Rahman, NA
Al-Ani, LA
Johan, M
Lu, J
Hashim, NM
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Journal Article
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Chiang Mai Journal of Science

The new potent nano-antioxidant of sulfur-containing butylated hydroxytoluene ligands (S-BHTLs)-conjugated with gold nanoparticles, Au-S-BHTLs, was synthesized by a conjugation of sulfur-containing ligands derived from BHT on the surface of gold nanoparticles (AuNPs). The in-house developing eight sulfur-containing BHT-ligands (S-BHTLs) were used for further study on functionalization with AuNPs and their biological activities. The antioxidant and cytotoxic abilities of eight types of Au-S-BHTLs such as compound Au-2a, Au-2b, Au-3a, Au-3b, Au-4a, Au-4b, Au-5a and Au-5b were tested by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and MTT assays against HT29 (human colorectal adenocarcinoma) and MCF7 (human breast adenocarcinoma). Based on the results, it was revealed that nanocomposites Au-5a and Au-3a attained over 50% lower IC values against free radical than the un-functionalized AuNPs. Meanwhile, nanocomposite Au-2b showed the highest cytotoxicity effect against both cancer cell lines. Selectivity and safety towards non-tumorgenic cell lines were also evaluated and proved superior selectivity index (SI) of all nanocomposites (SI > 2.00) against both cancer cells except Au-5b (SI = 1.87) against MCF7. Hence, the functionalization of S-BHTLs with gold nanoparticles has increased the selectivity of nanocomposites and enhanced the antioxidant potentials to become useful and promising anticancer agents. • 50

Gold nanoparticles; Au-S-BHTLs; DPPH; HT29; MCF7
Chiang Mai Journal of Science, 2021; 48(2) : 405-419
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