Rapid biofunctionalization of magnetic beads with function-spacer-lipid constructs
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KODE™ Technology is based on novel water-dispersible self-assembling molecules, called a functionspacer- lipids or KODE™ constructs (Figure 1) that are able to coat virtually any biological or non-biological surface with almost any biological or non-biological material [1-10]. The primary coating method of live cells, organisms, bacteria and viruses or solid surfaces (glass, metals, plastics, etc.) is achieved by simple contact with a solution containing one or more FSL KODE™ constructs. Upon contact the FSLs spontaneously and harmlessly create a stable and novel surface coating. Essentially the spontaneous self-assembling process is driven by the need of the constructs to “exclude water”. Because the constructs are able to bind to virtually any surface, be it hydrophobic or hydrophilic the mechanisms of action are multiple and complex and include hydrophobic interactions (via lipid tail), hydrophilic interactions (via the head group and spacer), micelle entrapment, encapsulation, bi/multi layer assembly, and other factors such as hydrogen bonding, van der Waals forces, electrostatic and ionic interactions and combinations of all the above on complex surfaces. To-date a large range of peptides, simple and complex carbohydrates (including sialic acids and hyaluronin), peptides, fluorescent markers, reactive functional groups, biotin (Figure 1), oligonucleotides,radiolabels, chelators, and other functional moieties have been created as FSL constructs [1-10]. The key advantages of KODE™ Technology over other conjugation techniques are that it allows the user to create bespoke novel surfaces on demand, and it can also harmlessly modify live cells to facilitate their attachment to beads. Because multiple different FSL constructs can be added simultaneously to a bead, and in a controlled manner (by simply altering relative concentrations of FSLs in the mix), users can build on the surface of the bead a variety of complex multi-ligand biofunctional surfaces. Furthermore, the technology is compatible with existing functionalized beads and would allow users to add further features, such as fluorescent labels, or other enhancing or blocking components.