AUT LibraryAUT
View Item 
  •   Open Research
  • Faculties
  • Faculty of Health and Environmental Sciences
  • School of Interprofessional Health Studies
  • View Item
  •   Open Research
  • Faculties
  • Faculty of Health and Environmental Sciences
  • School of Interprofessional Health Studies
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

The Impact of Curcumin-graphene Based Nanoformulation on Cellular Interaction and Redox-activated Apoptosis: An in Vitro Colon Cancer Study

Al-Ani, LA; Kadir, FA; Hashim, NM; Julkapli, NM; Seyfoddin, A; Lu, J; AlSaadi, MA; Yehye, WA
Thumbnail
View/Open
Journal article (1.570Mb)
Permanent link
http://hdl.handle.net/10292/13876
Metadata
Show full metadata
Abstract
Natural plants derivatives have gained enormous merits in cancer therapy applications upon formulation with nanomaterials. Curcumin, as a popular research focus has acquired such improvements surpassing its disadvantageous low bioavailability. To this point, the available research data had confirmed the importance of nanomaterial type in orienting cellular response and provoking different toxicological and death mechanisms that may range from physical membrane damage to intracellular changes. This in turn underlines the poorly studied field of nanoformulation interaction with cells as the key determinant in toxicology outcomes. In this work, curcumin-AuNPs-reduced graphene oxide nanocomposite (CAG) was implemented as a model, to study the impact on cellular membrane integrity and the possible redox changes using colon cancer in vitro cell lines (HT-29 and SW-948), representing drug-responsive and resistant subtypes. Morphological and biochemical methods of transmission electron microscopy (TEM), apoptosis assay, reactive oxygen species (ROS) and antioxidants glutathione and superoxide dismutase (GSH and SOD) levels were examined with consideration to suitable protocols and vital optimizations. TEM micrographs proved endocytic uptake with succeeding cytoplasm deposition, which unlike other nanomaterials studied previously, conserved membrane integrity allowing intracellular cytotoxic mechanism. Apoptosis was confirmed with gold-standard morphological features observed in micrographs, while redox parameters revealed a time-dependent increase in ROS accompanied with regressive GSH and SOD levels. Collectively, this work demonstrates the success of graphene as a platform for curcumin intracellular delivery and cytotoxicity, and further highlights the importance of suitable in vitro methods to be used for nanomaterial validation.
Keywords
Cancer research; Cell biology; Nanotechnology; Natural products; Anticancer; Cell membrane; Oxidative stress; Apoptosis
Date
2020
Source
Heliyon, 6(11), e05360.
Item Type
Journal Article
Publisher
Elsevier
DOI
10.1016/j.heliyon.2020.e05360
Publisher's Version
https://www.sciencedirect.com/science/article/pii/S2405844020322039?via%3Dihub
Rights Statement
© 2020 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).

Contact Us
  • Admin

Hosted by Tuwhera, an initiative of the Auckland University of Technology Library

 

 

Browse

Open ResearchTitlesAuthorsDateSchool of Interprofessional Health StudiesTitlesAuthorsDate

Alternative metrics

 

Statistics

For this itemFor all Open Research

Share

 
Follow @AUT_SC

Contact Us
  • Admin

Hosted by Tuwhera, an initiative of the Auckland University of Technology Library