Incidence in Plasma of Low Level Antibodies Against Three Xenotransplantation and Immunotherapeutic Glycan Antigens

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aut.relation.endpage87
aut.relation.issue4en_NZ
aut.relation.journalAIMS Allergy and Immunologyen_NZ
aut.relation.startpage75
aut.relation.volume4en_NZ
aut.researcherPerry, Elizabeth
dc.contributor.authorPerry, Hen_NZ
dc.contributor.authorRyzhov, Ien_NZ
dc.contributor.authorGalanina, Oen_NZ
dc.contributor.authorV Bovin, Nen_NZ
dc.contributor.authorM Henry, Sen_NZ
dc.date.accessioned2020-09-29T22:53:52Z
dc.date.available2020-09-29T22:53:52Z
dc.date.copyright2020en_NZ
dc.date.issued2020en_NZ
dc.description.abstractAntibodies against xeno-glycan antigens terminating with the saccharides Galα, GalNAcα and Rhaα are ubiquitous in human blood. Although originating as barriers to infection some of these naturally occurring complement-activating antibodies also contribute to disease processes, hinder xenotransplantation and have potential medical roles in immuno-oncotherapy. Because concentration of antibody is important in determining biological activity, there is a need to understand population variation in naturally occurring antibody levels, and to be able to rapidly and accurately determine levels in individuals. Xeno-glycan antigens in the form of function-spacer-lipid constructs were used to modify human red cells (kodecytes) to have on their surface micromolar equivalents of the xeno-glycan antigens Galα1-3Galβ1-4GlcNAc, GalNAcα1-3Galβ1-4GlcNAc and Rhaα. The methodology used was based on a previously validated kodecyte method used for quantifying IgM and IgG ABO human blood group antibodies in undiluted plasma. We tested plasma samples from 100 healthy individuals against these three different xeno-glycan kodecytes with each at three different loading concentrations of antigen to determine relative levels of these antibodies in human plasma. Sixty-one samples were also independently tested by enzyme immunoassay to correlate levels of anti-Galα. Results demonstrate independence between antibody specificities and substantial variation between individuals in levels of these antibodies, with >92% of the population having medium or high levels of at least one specificity. However, of particular importance was that 5–8% of the population had low levels of both IgM and IgG to at least one specificity and these individuals would probably have a poor immediate response when challenged by the corresponding antigen.
dc.identifier.citationAIMS Allergy and Immunology, 2020, 4(4): 75-87. doi: 10.3934/Allergy.2020007
dc.identifier.doi10.3934/allergy.2020007en_NZ
dc.identifier.issn2575-615Xen_NZ
dc.identifier.urihttps://hdl.handle.net/10292/13693
dc.languageenen_NZ
dc.publisherAmerican Institute of Mathematical Sciences (AIMS)en_NZ
dc.relation.urihttp://www.aimspress.com/article/10.3934/Allergy.2020007
dc.rights© 2020 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)
dc.rights.accessrightsOpenAccessen_NZ
dc.subjectXeno-glycan antigens; Function-spacer-lipid constructs; Kodecytes
dc.titleIncidence in Plasma of Low Level Antibodies Against Three Xenotransplantation and Immunotherapeutic Glycan Antigensen_NZ
dc.typeJournal Article
pubs.elements-id392409
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
pubs.organisational-data/AUT/Health & Environmental Science/Applied Science
pubs.organisational-data/AUT/Health & Environmental Science/School of Science
pubs.organisational-data/AUT/PBRF
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences
pubs.organisational-data/AUT/PBRF/PBRF Health and Environmental Sciences/HA Science 2018 PBRF
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