Variation in Gastric pH May Determine Kiwifruit's Effect on Functional Gi Disorder: An in Vitro Study

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aut.relation.endpage1500
aut.relation.issue4
aut.relation.startpage1488
aut.relation.volume6
aut.researcherYoung, Owen Archibald
dc.contributor.authorDonaldson, B
dc.contributor.authorRush, E
dc.contributor.authorYoung, O
dc.contributor.authorWinger, R
dc.date.accessioned2015-05-27T04:08:28Z
dc.date.available2015-05-27T04:08:28Z
dc.date.copyright2014-04
dc.date.issued2014-04
dc.description.abstractConsumption of kiwifruit is reported to relieve symptoms of functional gastrointestinal (GI) disorder. The effect may be related to the proteases in kiwifruit. This in vitro study aimed to measure protein hydrolysis due to kiwifruit protease under gastric and duodenal conditions. A sequence of experiments incubated meat protein, with and without kiwifruit, with varying concentrations of pepsin and hydrochloric acid, at 37 °C for 60 min over the pH range 1.3-6.2 to simulate gastric digestion. Duodenal digestion was simulated by a further 120 min incubation at pH 6.4. Protein digestion efficiency was determined by comparing Kjeldahl nitrogen in pre- and post-digests. Where acid and pepsin concentrations were optimal for peptic digestion, hydrolysis was 80% effective and addition of kiwifruit made little difference. When pH was increased to 3.1 and pepsin activity reduced, hydrolysis decreased by 75%; addition of kiwifruit to this milieu more than doubled protein hydrolysis. This in vitro study has shown, when gastric pH is elevated, the addition of kiwifruit can double the rate of hydrolysis of meat protein. This novel finding supports the hypothesis that consumption of kiwifruit with a meal can increase the rate of protein hydrolysis, which may explain how kiwifruit relieves functional GI disorder.
dc.identifier.citationNutrients 2014, 6, 1488-1500; doi:10.3390/nu6041488
dc.identifier.doi10.3390/nu6041488
dc.identifier.issn2072-6643
dc.identifier.urihttps://hdl.handle.net/10292/8803
dc.languageeng
dc.publisherMDPI AG
dc.relation.urihttp://dx.doi.org/10.3390/nu6041488
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.accessrightsOpenAccess
dc.subjectActinidia
dc.subjectAnimals
dc.subjectCattle
dc.subjectDietary proteins
dc.subjectDigestion
dc.subjectFruit
dc.subjectGastrointestinal diseases
dc.subjectHydrogen-Ion concentration
dc.subjectHydrolysis
dc.subjectMeat
dc.subjectModels
dc.subjectBiological
dc.subjectPeptide hydrolases
dc.titleVariation in Gastric pH May Determine Kiwifruit's Effect on Functional Gi Disorder: An in Vitro Study
dc.typeJournal Article
pubs.elements-id165439
pubs.organisational-data/AUT
pubs.organisational-data/AUT/Health & Environmental Science
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