Structure of Supramers Formed by the Amphiphile Biotin‐CMG‐DOPE

Date
2020-06
Authors
Zalygin, A
Solovyeva, D
Vaskan, I
Henry, S
Schaefer, M
Volynsky, P
Tuzikov, A
Korchagina, E
Ryzhov, I
Nizovtsev, A
Supervisor
Item type
Journal Article
Degree name
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract

The synthetic function-spacer-lipid (FSL) amphiphile biotinCMG-DOPE is widely used for delicate ligation of living cells with biotin residues under physiological conditions. Since this molecule has an “apolar-polar-hydrophobic” gemini structure, the supramolecular organization is expected to differ significantly from the classical micelle. Its organization is investigated with experimental methods and molecular dynamics simulations (MDS). Although the linear length of a single biotin-CMGDOPE molecule is 9.5 nm, the size of the dominant supramer globule is only 14.6 nm. Investigations found that while the DOPE tails form a hydrophobic core, the polar CMG spacer folds back upon itself and predominantly places the biotin reside inside the globule or planar layer. MDS demonstrates that <10% of biotin residues on the highly water dispersible globules and only 1% of biotin residues in layer coatings are in an linear conformation and exposing biotin into the aqueous medium. This explains why in biotin-CMG-DOPE apolar biotin residues both in water dispersible globules and coatings on solid surfaces are still capable of interacting with streptavidin.

Description
Keywords
Amphiphiles; Function-spacer-lipid; Biotin; Molecular dynamics; Supramers; Molecular dynamics simulations
Source
ChemistryOpen 2020, 9, 641–648.
Rights statement
© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.